| 1. | A late baculoviral transcription initiation motif ataag was found 65 nt upstream of the putative translational start site and a polyadenylation signal aataaa was identified 14 nt downstream of the taa stop codon
在其起始密码子上游- 65nt发现杆状病毒晚期启动子转录起始信号ataag ，在其终止密码子下游- 14nt发现polya加尾信号aataaa 。 |
| 2. | Activity of each construct was determined . the basal promoter was located at about 60bp up stream of the transcription initiation site . it contains a tata box at - 33bp which is required for the transcription initiation
基因的基本启动子元件位于转录起始位点上游约60bp ，其中含有一个位于- 33bp处的tatabox ，它对于转录起始起着重要作用。 |
| 3. | 5 ' race analysis indicated that ha 122 transcripts start predominantly in the consensus major late transcription initiation motif rtaag around 47nt upstream of the putative translation start codon with a minor start at position - 89
5 ’ race ( rapidamplificationofcdnaend )分析结果表明ha122基因的转录主要从翻译起始密码子上游第47个碱基起始，该处有一个晚期转录信号taag 。 |
| 4. | It is well documented that they jointly take part in gene transcription that led by rna polymerase ii . however , whether baf complex and nf1 / ctf are also involved in the formation of rna polymerase ii - mediated transcription initiation complex is still uncertain , and a number of the currently proposed models still lack the support of experimental data
哺乳动物swi snf ( baf )复合物和转录因子( nf1 ctf )都是转录活动的重要调控因子，许多实验证实，它们共同参与了基因转录调控活动。 baf复合物与转录因子nf1 ctf是否一同参加了由rna聚合酶介导的基因转录起始复合物的形成，目前还没有比较一致的认识，已有的模型仍缺乏实验支持。 |
| 5. | 3 , by immunofluoresent microscopy , we also found that brg1 did not clearly co - localize with rna polymerase ii small subunit b6 , but partially co - localized with rna polymerase ii small subunit b8 ; nf1 / ctf clearly co - localized with rna polymerase ii small subunit b6 and partially with b8 . these data implies that brg1 may be partially associated to b8 and nf1 / ctf may be involved in the formation of transcription initiation complex through associating to brg1 and b6
3 、通过兔疫荧光实验我们还发现， brg和rna聚合酶d亚基b6之间没有明显共定位现象，与rna聚合酶11小亚基bs有部分的共定位现象：呷1儿仆与删a聚合酶小亚基孤有比较明显的共定位，与rna聚合酶11小亚基bs有部分的共定位。 |
